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Techniques

  • Cell Biology:
    Cell culture
    Live cell imaging
    GFP / RFP
    Transfections
  • Microscopy:
    • Electron microscopy
      Negative contrasting of proteins and protein complexes
      EM of cells and tissues
      Scanning EM
    • Light microscopy
      Histological enzyme stains
      Histological stains
      Double- and triple-immuno-fluorescence
      Confocal laser-scanning microscopy
      Living cell fluorescence microscopy
  • Molecular Biology:
    Cloning of long cDNAs
    RT-PCR, RACE
    Site-directed mutagenesis
    Gene structure analysis
    Yeast two hybrid
    Epitope-tagging
  • Protein Chemistry:
    Expression and purification of recombinant proteins
    Folding- and structure analysis
    Chemical cross-linking
    Protein-interaction assays
    Actin-cosedimentation
    Protein-phosphorylation
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