equipment-en
Equipment
Optical sectioning of fluorescence-labelled cells and tissue sections
Multi-Channel fluorescence imaging
Generation of image stacks for 3D reconstruction of samples
Automated scanning of large sample areas (tile scanning)
Live cell imaging over extended periods of time (incubation chambers)
Assays to measure protein dynamics and protein-protein-interactions: FRAP, FRET, BiFC
| LSM 710 (Zeiss) | IZB | CLSM with spectral detection; large incubation chamber for long-time live cell imaging; FRAP-, FRET- and BiFC-analytics |
| Cell Observer SD (Zeiss) | IZB | fast and highly sensitive system with incubation system for live cell imaging; FRAP-, FRET- und BiFC-analytics |
| LSM 900 (Zeiss) | IZB | high resolution CLSM with Airy Scan detector and incubation system for live cell imaging; FRAP-, FRET- und BiFC-analytics |
| SP8 Lightning (Leica) | IZMB | spectral CLSM with with GaAsP detector; FRET- and BiFC-analytics |
| Ti2-E STORM 5.0 (Nikon) | IG | Inverted microscope equipped with perfect focus system, N-STORM kit
and H-TIRF controller for Stochastical Optical Reconstruction (STORM)
microscopy |
Imaging of fluorescence-labelled living or chemically fixed cells
Imaging of tissue sections after staining with histologic dyes (bright field mode)
| Axio-Imager.M1 (Zeiss) | IZB | upright system; dual camera solution for brightfield and fluorescence imaging |
| Axio-Observer.Z1 (Zeiss) | IZB | inverted system with Apotom 2 for generating optical sections |
| Axio-Imager.M2 (Zeiss) | Nees | upright system |
| BZ-9000E (Keyence) | IG | inverted system |
| Axiophot (Zeiss) | IG | also equipped with 63x air objective |
| DM 5500 (Leica) | IG | upright system |
Automated imaging of microscopic slides
| VHX-1000 (Keyence) | Nees | stacking of three-dimensional samples |
| Slide Scanner VS200 (Olympus) | IEZ | digital slide scanner for capturing high-resolution images of slides |
Cryo-microtomes for cutting semi-thin sections for fluorescence microscopy
Microtomes to generate sections of paraffin-embedded tissues for subsequent histological staining
| Vibratome/Microtome for histology (Micron) | IEZ | cutting sections from paraffin-embedded material |
| Vibratome for histology (Jung) | IZMB | agarose sections for light microscopy |
| microtomes (Jung) | IZMB | paraffin and Stedman wax sections for light microscopy |
| Cryocut CM 3050 S (Leica) | IZB | cutting semi thin sections of frozen tissues for immunofluorescence microscopy |
| RM 2165 (Leica) | IZB | cutting sections from paraffin-embedded material for histological staining |
Scanning EM: Imaging of surfaces with BSE- and SE-detectors yielding material contrast and topographic data, respectively
Transmission EM: Imaging of ultra-thin sections and single molecules
Immuno-gold detection
| SEM Verios 460L (FEI) | IZB | ultra-high-resolution scanning EM with STEM-detector for TEM studies; SE-detectors for topography and BSE-detector for material contrast; MAPS-Software for imaging large samples ("Tile and Stitch") |
Microtomes to cut ultra-thin sections from resin-embedded samples
Sputter coaters and high-vacuum evaporators to prepare samples for SEM or TEM
Critical point dryers for preparing SEM samples
| Ultracut EM UC7 (Leica) | IZB | ultramicrotomes for cutting ultra-thin resin sections for TEM |
| Ultracut S, UC6 (Leica) | IEZ | ultramicrotomes for cutting ultra-thin resin sections for TEM |
| Ultracut S (Reichert) | IZMB | ultramicrotome for cutting ultra-thin resin sections for TEM |
| 208 HR (Cressington) | IZB | sputter coater device for creating an ultra-thin layer of platin/palladium yielding stability and conductivity for SEM samples |
| EM ACE 600 (Leica) | IZB | high-vacuum evaporation system for generating ultra-fine layers of carbon and platin for contrast and stability for TEM-samples |
| CPD 030 (BAL-TEC) | IZB IEZ |
critical-point dryers for SEM samples |
Measuring and imaging details of surface topography
| AFM: Dimension 3100 (Veeco/Bruker) NI: Hysitron (TriboScope) |
IZ | atomic force microscope (AFM) for imaging and measuring surface
topography, equipped with nanoindenter (NI) for local measurement of
E-module and hardness |
Non-invasive tomography of small, fresh or dried samples to identify histology and micromorphology
| Skyscan µCT 1272 (Bruker) | IEZ | µComputertomograph (resolution 0.4 µm) |
